Publication: Clifford Luke, Washington University in St. Louis
Clifford Luke at Washington University School of Medicine in St. Louis used vivoChip to investigate regulation of cell survival vs death in the face of physiological stress in their recent paper "Lysoptosis is an evolutionarily conserved cell death pathway moderated by intracellular serpins" published in Nature Communications Biology.
vivoChip was used to conduct time-lapse imaging of lysosomal membrane permeabilization (LMP) as indicated by loss of fluorescent dextran. Worms null for the cysteine protease inhibitor, srp-6 showed a significant enhancement of lysosomal permeability upon hypotonic stress. The vivoChip allowed high-resolution 3D confocal imaging of the same animals every 15 m over 4 hours.
