November 21, 2019

Customer testimonials and applications


Clifford Luke, Washington University in St. Louis

Clifford Luke at Washington University School of Medicine in St. Louis used vivoChip to investigate regulation of cell survival vs death in the face of physiological stress in their recent paper "Lysoptosis is an evolutionarily conserved cell death pathway moderated by intracellular serpins" published in Nature Communications Biology.

vivoChip was used to conduct time-lapse imaging of lysosomal membrane permeabilization (LMP) as indicated by loss of fluorescent dextran. Worms null for the cysteine protease inhibitor, srp-6 showed a significant  enhancement of lysosomal permeability upon hypotonic stress. The vivoChip allowed high-resolution 3D confocal imaging of the same animals every 15 m over 4 hours.


Pierce-Shimomura Lab, University of Texas at Austin

vivoChip was recently used by Wisath Sae-Lee from the Jon Pierce laboratory at the University of Texas at Austin in their recent paper "APP-Induced Patterned Neurodegeneration Is Exacerbated by APOE4 in Caenorhabditis elegans" published in G3.

G3 figure

vivoChip was used to score HSN neurodegeneration and found it increased when APOE4 was overexpressed with human APP, but not a related C. elegans protein APL-1. It suggests interaction between APP and APOE4 is important in the development of Alzheimer's

"vivoVerse deliver on their promises, and have enabled our lab to perform high-throughput imaging in C. elegans. Their system is very easy to set up and use, the worms are very robustly oriented, and the chip is suitable for even high-resolution imaging!"

Steven Ban,
Saul Kato Lab, UCSF

neuroPAL worms

NeuroPAL C. elegans (polychromatic, multi-neuronal marker gene) in a vivoChip. Imaging conditions: 40X, 1.3NA, volumetric image taken over 50µm, maximum intensity projected. Image courtesy of Steven Ban, UCSF

“We find that vivoChip is a very intelligent system, easy to use and very fast. It is really impressive, and we were so happy to see these beautiful pictures that we took using wide-field and spinning disc microscopes.”

Claudia Maios and Gilles Tossing
Alex Parker Lab, University of Montreal

Dr. Parker’s lab members are using the vivoVerse vivoChip platform to immobilize age-synchronized adult animals in the device. Gilles was able to visualize up to 9 days adult C. elegans expressing fluorescent markers and interested to use the platform for long-term imaging experiments.

unc-47 worms

Fluorescence images of unc-47::mCherry animals immobilized inside the vivoChip. Widefield and spinning disc images of 8 days adult animals were captured to score for individual neurons and their connections in a high-resolution time-lapse experiment. Image courtesy of Gilles Tossing, CHUM.

“Day 2 of using the vivoCube from vivoVerse ! Still learning some tricks to improve/perfect the set-up process, but super excited to do science with this new toy! Huge thanks to vivoVerse support for getting us started.”

Joseph Liang,
Catharine Rankin lab, University of British Columbia

pdr-1 worms

pdr-1(tm1598), pdat1::GFP C. elegans (D1). These are a part of an imaging experiment that investigates whether there is more neurodegeneration as these worms age. pdr-1 is the worm homolog for human PRKN, a gene implicated in Parkinson’s Disease. Image courtesy of Joseph Liang, UBC

“The vivoCube and vivoChip system has revolutionized my imaging days! It’s easy to set up, fits nicely into our space, and is very user-friendly. And it’s so fast! I can image and score a full chip in about 40 minutes!”

Lotti Brose,
Pierce-Shimomura Lab, University of Texas at Austin

Also from the Jon Pierce laboratory at the University of Texas at Austin is Dr. Lotti Brose. She has been using the vivoVerse (formerly Newormics) D1-D5 vivoChips to study HSN neurons (part of the C. elegans egg laying circuitry) in D1-D3 animals. Lotti has been particularly happy with the speed at which worms can be imaged, and is able to reuse her vivoChips multiple times for different batches of worms.

“I found the L4-YA chip from vivoVerse extremely effective in immobilizing L4s and adults for live imaging even without supplementing the system with any anesthetics. The imaged worms also seemed to be in good condition when the imaging session was concluded (approximately one hour), which is great in cases where the worms need to be recovered for propagation.”

Nikhil Mishra,
Barbara Conradt Lab, LMU Munich

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