Clifford Luke, Washington University in St. Louis
Clifford Luke at Washington University School of Medicine in St. Louis used vivoChip to investigate regulation of cell survival vs death in the face of physiological stress in their recent paper "Lysoptosis is an evolutionarily conserved cell death pathway moderated by intracellular serpins" published in Nature Communications Biology.
vivoChip was used to conduct time-lapse imaging of lysosomal membrane permeabilization (LMP) as indicated by loss of fluorescent dextran. Worms null for the cysteine protease inhibitor, srp-6 showed a significant enhancement of lysosomal permeability upon hypotonic stress. The vivoChip allowed high-resolution 3D confocal imaging of the same animals every 15 m over 4 hours.
Pierce-Shimomura Lab, University of Texas at Austin
vivoChip was recently used by Wisath Sae-Lee from the Jon Pierce laboratory at the University of Texas at Austin in their recent paper "APP-Induced Patterned Neurodegeneration Is Exacerbated by APOE4 in Caenorhabditis elegans" published in G3.
vivoChip was used to score HSN neurodegeneration and found it increased when APOE4 was overexpressed with human APP, but not a related C. elegans protein APL-1. It suggests interaction between APP and APOE4 is important in the development of Alzheimer's
NeuroPAL C. elegans (polychromatic, multi-neuronal marker gene) in a vivoChip. Imaging conditions: 40X, 1.3NA, volumetric image taken over 50µm, maximum intensity projected. Image courtesy of Steven Ban, UCSF
Dr. Parker’s lab members are using the vivoVerse vivoChip platform to immobilize age-synchronized adult animals in the device. Gilles was able to visualize up to 9 days adult C. elegans expressing fluorescent markers and interested to use the platform for long-term imaging experiments.
Fluorescence images of unc-47::mCherry animals immobilized inside the vivoChip. Widefield and spinning disc images of 8 days adult animals were captured to score for individual neurons and their connections in a high-resolution time-lapse experiment. Image courtesy of Gilles Tossing, CHUM.
pdr-1(tm1598), pdat1::GFP C. elegans (D1). These are a part of an imaging experiment that investigates whether there is more neurodegeneration as these worms age. pdr-1 is the worm homolog for human PRKN, a gene implicated in Parkinson’s Disease. Image courtesy of Joseph Liang, UBC
Also from the Jon Pierce laboratory at the University of Texas at Austin is Dr. Lotti Brose. She has been using the vivoVerse (formerly Newormics) D1-D5 vivoChips to study HSN neurons (part of the C. elegans egg laying circuitry) in D1-D3 animals. Lotti has been particularly happy with the speed at which worms can be imaged, and is able to reuse her vivoChips multiple times for different batches of worms.